|Search for predicted microRNA targets in mammals||[Go to the newest version of TargetScanHuman]|
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* broadly conserved = conserved across most vertebrates, usually to zebrafish
conserved = conserved across most mammals, but usually not beyond placental mammals
TargetScan predicts biological targets of miRNAs by searching for the presence of, , and sites that match the of each miRNA (Lewis et al., 2005). As an option, only conserved sites are predicted. Also identified are sites with mismatches in the seed region that are compensated by conserved 3' pairing (Friedman et al., 2009) and centered sites (Shin et al., 2010). In mammals, predictions are ranked based on the predicted efficacy of targeting as calculated using cumulative weighted context++ scores of the sites (Agarwal et al., 2015). As an option, predictions are also ranked by their probability of conserved targeting (PCT, Friedman et al., 2009). TargetScanHuman considers matches to human 3' UTRs and their orthologs, as defined by UCSC whole-genome alignments. Conserved targeting has also been detected within open reading frames (ORFs). A listing of these ORF sites can be found at the bottom of Supplemental Table 2 of Lewis et al., 2005.
This search page of TargetScan Release 7.0 retrieves predicted regulatory targets of mammalian microRNAs. Many targets are the same as those presented in previous versions of the TargetScan site (Releases 2.0, 2.1, 3.0, 3.1, 4.0 - 4.2, 5.0 - 5.2, and 6.0 - 6.2 (Lewis et al., 2005; Grimson et al., 2007; Friedman et al., 2009; García et al., 2011). Compared to previous releases, Release 7 uses an improved method to predict targeting efficacy (the context++ model, Agarwal et al., 2015), uses 3' UTR profiles that indicate the fraction of mRNA containing each site (Nam et al., 2014), and uses updated miRNA families (Agarwal et al., 2015).
An introduction to microRNAs (iBioseminar)
Frequently Asked Questions (FAQs)
More information about Release 7.0
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